Yes,  had this happen more than once with T-Spot.   Per their website:

What should I do with a borderline T-SPOT.TB test result?

Borderline results are clinically interpretable and should be followed. Retesting by collecting another sample is recommended. In a study of U.S. healthcare workers, 23% of subjects with a borderline test result retested as positive, suggesting the borderline category is useful in maintaining test sensitivity. Upon retesting, if the test result remains borderline, other diagnostic tests and/or epidemiologic information should be used to help determine the TB infection status of the patient.1

1. King TC, Upfal M, Gottlieb A, et al. T-SPOT.TB Interferon- Release Assay Performance in Healthcare Worker Screening at Nineteen U.S. Hospitals. Am J Respir Crit Care Med. 2015;192(3):367-373. doi:10.1164/rccm.201501-0199OC.


Christy Rossiter, RN, CMSRN

Employee Health Coordinator




700 Lawn Ave

Sellersville, PA 18960

Telephone: 215-453-4016

Fax: 215-453-4748



From: MCOH-EH [] On Behalf Of Senior, Cathy (Employee Health)
Sent: Monday, April 25, 2016 12:12 PM
Subject: [MCOH-EH] IGRA


We use Interferon Gamma Release Assays ( IGRA) to test for tuberculosis on all new employees.  I have one new employee that I have tested twice (with approx one month in between testing) and both times the results were borderline.  Has anyone else had this come up and if so what did you do next?  This employee has no symptoms and no high risk activity such as traveling outside the country or having contact with anyone with known Tb.



Cathy Senior RN BSN CDE

Employee Health Director

Penn Highlands DuBois

100 Hospital Avenue

P.O. Box 447

DuBois Pa 15801

Phone 814-375-3392

Fax 814-372-2610


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